abstract：:The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...

journal_title：Cytotechnology

authors： Castagnola P,Cancedda R

更新日期：1991-02-01 00:00:00

abstract：:The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...

journal_title：Cytotechnology

authors： Monti D,Troiano L,Tropea F,Grassilli E,Cossarizza A,Barozzi D,Bettuzzi S,Corti A,Franceschi C

更新日期：1991-02-01 00:00:00

abstract：:The application of image analysis methods to conventional thin sections for electron microscopy to analyze the chromatin arrangement are quite limited. We developed a method which utilizes freeze-fractured samples; the results indicate that the method is suitable for identifying the changes in the chromatin arrangemen...

journal_title：Cytotechnology

authors： Maraldi NM,Marinelli F,Squarzoni S,Santi S,Barbieri M

更新日期：1991-02-01 00:00:00

abstract：:Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...

journal_title：Cytotechnology

authors： Cinelli S,Falezza A,Meli C,Ciliutti P,Vericat JA

更新日期：1991-01-01 00:00:00

abstract：:We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...

journal_title：Cytotechnology

authors： Kelly D,Campbell WJ,Tiesman J,Rizzino A

更新日期：1990-11-01 00:00:00

abstract：:A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collagen/chitosan mixture were loaded to the cell chamber and were allowed to form gel inside. Contraction of the cel...

journal_title：Cytotechnology

authors： Scholz M,Hu WS

更新日期：1990-09-01 00:00:00

abstract：:The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...

journal_title：Cytotechnology

authors： Iwamoto K,Shintani Y,Sasada R,Honjo T,Kitano K

更新日期：1990-07-01 00:00:00

abstract：:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...

journal_title：Cytotechnology

authors： Borth N,Steindl F,Weigang F,Reiter M,Katinger H

更新日期：1990-05-01 00:00:00

abstract：:Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to serum-free medium ...

journal_title：Cytotechnology

authors： Gammon MC,Banas MH,Boccumini LE,Sigal NH,Zweerink HJ

更新日期：1990-01-01 00:00:00

abstract：:Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...

journal_title：Cytotechnology

authors： Macdonald RG

更新日期：1989-12-01 00:00:00

abstract：:Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effect...

journal_title：Cytotechnology

authors： Marquis CP,Low KS,Barford JP,Harbour C

更新日期：1989-08-01 00:00:00

abstract：:Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...

journal_title：Cytotechnology

authors： Sen S,Srienc F,Hu WS

更新日期：1989-05-01 00:00:00

abstract：:In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture co...

journal_title：Cytotechnology

authors： Kohgo Y,Sakamaki S,Kanisawa Y,Nojiri S,Ueno Y,Itoh Y,Takahashi M,Sasagawa Y,Hosoi S,Sato S,Niitsu Y

更新日期：1989-02-01 00:00:00

abstract：:Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000 SW and DEAE-5PW co...

journal_title：Cytotechnology

authors： Miyata M,Murakami H,Hashizume S,Yamada K,Omura H

更新日期：1988-11-01 00:00:00

abstract：:We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formatio...

journal_title：Cytotechnology

authors： Thomou H,Sambani C,Kitsiou P,Politis G

更新日期：1988-07-01 00:00:00

abstract：:Anin vitro system is described which uses human diploid cells derived from the periodontal ligament. It simulates the clinical situation and is suitable for rapid cytotoxicity screening of dental materials. It can also be adapted to study various factors influencing biocompatibility. ...

journal_title：Cytotechnology

authors： Adams AM,Soames JV,Searle RF

更新日期：1988-02-01 00:00:00

abstract：:The low-metastatic RSV-transformed fibrosarcoma line B77-3T3 and its metastatic variant AA12, selected in vitro, have been analysed by blot and in situ hybridization with v-src and murine c-myc specific probes in order to detect molecular rearrangements underlying the transition from the low-metastatic to the high-met...

journal_title：Cytotechnology

authors： Doneda L,Custode P,Morghen Cde G,Larizza L

更新日期：1987-10-01 00:00:00